Escola Paulista de Medicina
Postgraduate Program in Pharmacology

Anthelmintic effects of a cationic toxin from a South American rattlesnake venom

2.toxicon

Dal Mas, C., Moreira, J.T., Pinto, S., Monte, G.G., Nering, M.B., Oliveira, E.B., Gazarini, M.L., Mori, M.A., Hayashi, M.A.F.

Despite the unquestionable importance of the highly cationic feature of several small polypeptides with high content of positively charged amino acids for their biological activities, positively charged peptides do not necessarily have the capacity to cross the cell membranes. Interestingly, we found that crotamine, a positively charged amphiphilic peptide from the South American rattlesnake venom, has a unique cell-penetrating property with affinity for acidic vesicles, besides a well-characterized antimicrobial and antitumoral activities. In spite of a remarkable in vitro antifungal activity of crotamine against Candida spp., no significant effect of this peptide could be observed in the course of Candida albicans and Candida krusei infection on Caenorhabditis elegans asssed in vivo. These experiments, in which the nematode C. elegans was used as a living host, suggested, however, the potential anthelmintic activity of crotamine because of its uptake by the worms and accumulation in their acidic compartments. As described in the present work, this lysosomotropic property is consistent with a previously proposed mechanism of toxicity of crotamine on mammalian tumoral cell lines. This study also allowed us to propose the cationic peptides with lysosomotropic property, as crotamine, as a potential new class of anthelmentics with ability to overcome the challenging problems of drug resistance.

 

Artigos Relacionados

28 Sep 2021 17:17

Wuo-Silva R, Fukushiro-Lopes DF, Fialho BP, Hollais AW, Santos-Baldaia R, Marinho EAV, Mári-Kawamoto [ ... ]

Artigos Cientificos
28 Sep 2021 16:53

Antunes F, Pereira GJS, Saito RF, Buri MV, Gagliardi M, Bincoletto C, Chammas R, Fimia GM, Piacentini [ ... ]

Artigos Cientificos


© 2013 a 2021 - Universidade Federal de São Paulo - Unifesp
Instituto de Farmacologia e Biologia Molecular - Rua Três de Maio, 100 - Vila Clementino, São Paulo - SP - ppg.farmacologia@unifesp.br